LOCUS       40924_40512        4790 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pSK(+)PIBT 14, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Transformation of Ulva mutabilis Foyn with plasmids integrating into
            the chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-2007) Biochemistry, Genetics and Microbiology, 
            Division of Genetics, University of Regensburg, Universitaetsstr. 
            31, Regensburg, Bavaria 93053, Germany
REFERENCE   3  (bases 1 to 4790)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4790)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-OCT-2007) Biochemistry, Genetics and Microbiology, Division of 
            Genetics, University of Regensburg, Universitaetsstr. 31, 
            Regensburg, Bavaria 93053, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..546
                     /label=Ulva mutabilis RbcS promoter
                     /note="Ulva mutabilis RbcS promoter"
                     /regulatory_class="promoter"
     intron          555..1051
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
     CDS             1050..1415
                     /label=BleoR
                     /note="antibiotic-binding protein"
     regulatory      1419..1807
                     /label=Ulva mutabilis RbcS terminator
                     /note="Ulva mutabilis RbcS terminator"
                     /regulatory_class="terminator"
     promoter        complement(1832..1850)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1857..1873)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2018..2473
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2502..2606
                     /label=AmpR promoter
     CDS             2607..3464
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3638..4226
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4514..4535
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4550..4580
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4588..4604
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4612..4628
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4649..4667
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     4704..4720
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4754..4770)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgttgttcgt acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag
       61 tccagatacc atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac
      121 tgcactaatc tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga
      181 gtactggcac aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg
      241 ctccaataat gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac
      301 gtgccggggt tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg
      361 tgtggcacga gatatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca
      421 tgccgctcga gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct
      481 cctgtctctg ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca
      541 gcagacatgg ccaagtgcgc atcgcgtgtc aatgctggcg ctgctgacgt ccgctgccta
      601 aactgcatga aagctcctgc agtcgcaagt agtcctttta acttgttgta tcggaacaaa
      661 tggttgtagt ctgtgccgtg tgacgatctt gagccgcctg gtcggcaggt ccaatgtcgg
      721 cctggaccct gcacaccctg cccaccctgc cgctgctcac ccgttcacga ccccacaggt
      781 gctcgcagca gtaattgtcg atagatagca actgtgttct gactattctt tgaccttccg
      841 catttgggac tgcggcggca cacctactat ccgcaatgca caccctggtg ctggtactgc
      901 ctgcctttcc gcgcgagccc acaccatgca ggcgcatagt aatggactgt gattgattta
      961 ggcaactgta tgaaacctct tcatcgtgcc ttggttgctt gcgataccct cacgtgagtt
     1021 catatgacca acgctggcac gacccctgca ggatcaccag cgccgttccg gtgctcaccg
     1081 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     1141 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     1201 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     1261 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     1321 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     1381 gcaactgcgt gcacttcgtg gccgaggagc aggactaagc agtcaggcag agtcgttgtt
     1441 gggtgtatgg ttgcacaaag ctagacctcg agctggtcgg ttagaaagag atgcttgtgc
     1501 gactggatgt aacaggcaca atccgaggaa aatgcaacag aaacgttatg atgtaacatc
     1561 tgagcacagt caataaaatt gcatgttgta tgccgcacac aaaagtgttg ttaaacatgc
     1621 gatgtggttg tcaaatcatt attcgcacaa ccatggaccg aacctctggc acgctgcata
     1681 cgctgtattc attcacatca tgttttctac ttgtcacatg ctctgcacca agtaactccg
     1741 attagttagg ttcgcacatg tcatcggagt ggaccacttc gaatcatttg gttcgaagac
     1801 aaaccgggaa ttggcccggt acccaattcg ccctatagtg agtcgtatta caattcactg
     1861 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     1921 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     1981 tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta
     2041 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2101 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2161 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2221 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     2281 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     2341 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     2401 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     2461 acgtttacaa tttcccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2521 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2581 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2641 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2701 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2761 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2821 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2881 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2941 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     3001 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     3061 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     3121 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     3181 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     3241 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3301 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3361 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3421 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3481 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3541 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3601 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3661 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3721 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3781 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3841 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3901 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3961 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4021 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4081 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4141 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4201 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4261 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4321 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4381 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4441 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4501 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     4561 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     4621 gctatgacca tgattacgcc aagctcggaa ttaaccctca ctaaagggaa caaaagctgg
     4681 agctccaccg cggtggcggc cgctctagaa ctagtggatc ccccgggctg caggaattcg
     4741 atatcaagct tatcgatacc gtcgacctcg agggggggca attcccatgg
//
